Publications

365 entries « 7 of 8 »

2005

Frankel, B A; Kruger, R G; Robinson, D E; Kelleher, N L; McCafferty, D G

Staphylococcus aureus sortase transpeptidase SrtA: insight into the kinetic mechanism and evidence for a reverse protonation catalytic mechanism Journal Article

Biochemistry, 44 (33), pp. 11188-200, 2005, ISSN: 0006-2960 (Print) 0006-2960 (Linking).

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Garneau, S; Dorrestein, P C; Kelleher, N L; Walsh, C T

Characterization of the formation of the pyrrole moiety during clorobiocin and coumermycin A1 biosynthesis Journal Article

Biochemistry, 44 (8), pp. 2770-80, 2005, ISSN: 0006-2960 (Print) 0006-2960 (Linking).

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Gatto, Jr. G J; McLoughlin, S M; Kelleher, N L; Walsh, C T

Elucidating the substrate specificity and condensation domain activity of FkbP, the FK520 pipecolate-incorporating enzyme Journal Article

Biochemistry, 44 (16), pp. 5993-6002, 2005, ISSN: 0006-2960 (Print) 0006-2960 (Linking).

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Kelleher, N L; Hicks, L M

Contemporary mass spectrometry for the direct detection of enzyme intermediates Journal Article

Curr Opin Chem Biol, 9 (5), pp. 424-30, 2005, ISSN: 1367-5931 (Print) 1367-5931 (Linking).

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McLoughlin, S M; Kelleher, N L

Monitoring multiple active sites on thiotemplate enzymes in parallel: a molecular movie of yersiniabactin bioassembly Journal Article

J Am Chem Soc, 127 (43), pp. 14984-5, 2005, ISSN: 0002-7863 (Print) 0002-7863 (Linking).

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McLoughlin, S M; Mazur, M T; Miller, L M; Yin, J; Liu, F; Walsh, C T; Kelleher, N L

Chemoenzymatic approaches for streamlined detection of active site modifications on thiotemplate assembly lines using mass spectrometry Journal Article

Biochemistry, 44 (43), pp. 14159-69, 2005, ISSN: 0006-2960 (Print) 0006-2960 (Linking).

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Meng, F; Forbes, A J; Miller, L M; Kelleher, N L

Detection and localization of protein modifications by high resolution tandem mass spectrometry Journal Article

Mass Spectrom Rev, 24 (2), pp. 126-34, 2005, ISSN: 0277-7037 (Print) 0277-7037 (Linking).

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Miller, L M; Mazur, M T; McLoughlin, S M; Kelleher, N L

Parallel interrogation of covalent intermediates in the biosynthesis of gramicidin S using high-resolution mass spectrometry Journal Article

Protein Sci, 14 (10), pp. 2702-12, 2005, ISSN: 0961-8368 (Print) 0961-8368 (Linking).

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Roth, M J; Forbes, A J; Boyne, 2nd M T; Kim, Y B; Robinson, D E; Kelleher, N L

Precise and parallel characterization of coding polymorphisms, alternative splicing, and modifications in human proteins by mass spectrometry Journal Article

Mol Cell Proteomics, 4 (7), pp. 1002-8, 2005, ISSN: 1535-9476 (Print) 1535-9476 (Linking).

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Lanen, Van S G; Dorrestein, P C; Christenson, S D; Liu, W; Ju, J; Kelleher, N L; Shen, B

Biosynthesis of the beta-amino acid moiety of the enediyne antitumor antibiotic C-1027 featuring beta-amino acyl-S-carrier protein intermediates Journal Article

J Am Chem Soc, 127 (33), pp. 11594-5, 2005, ISSN: 0002-7863 (Print) 0002-7863 (Linking).

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Yin, J; Straight, P D; McLoughlin, S M; Zhou, Z; Lin, A J; Golan, D E; Kelleher, N L; Kolter, R; Walsh, C T

Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase Journal Article

Proc Natl Acad Sci U S A, 102 (44), pp. 15815-20, 2005, ISSN: 0027-8424 (Print) 0027-8424 (Linking).

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Zabrouskov, V; Senko, M W; Du, Y; Leduc, R D; Kelleher, N L

New and automated MSn approaches for top-down identification of modified proteins Journal Article

J Am Soc Mass Spectrom, 16 (12), pp. 2027-38, 2005, ISSN: 1044-0305 (Print) 1044-0305 (Linking).

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McLoughlin, S M; Mazur, M T; Miller, L M; Yin, J; Liu, F; Walsh, C T; Kelleher, N L

Chemoenzymatic approaches for streamlined detection of active site modifications on thiotemplate assembly lines using mass spectrometry Journal Article

Biochemistry, 44 (43), pp. 14159-14169, 2005, ISSN: 0006-2960.

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2004

Du, Y; Meng, F; Patrie, S M; Miller, L M; Kelleher, N L

Improved molecular weight-based processing of intact proteins for interrogation by quadrupole-enhanced FT MS/MS Journal Article

J Proteome Res, 3 (4), pp. 801-6, 2004, ISSN: 1535-3893 (Print) 1535-3893 (Linking).

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Forbes, A J; Patrie, S M; Taylor, G K; Kim, Y B; Jiang, L; Kelleher, N L

Targeted analysis and discovery of posttranslational modifications in proteins from methanogenic archaea by top-down MS Journal Article

Proc Natl Acad Sci U S A, 101 (9), pp. 2678-83, 2004, ISSN: 0027-8424 (Print) 0027-8424 (Linking).

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Hicks, L M; O'Connor, S E; Mazur, M T; Walsh, C T; Kelleher, N L

Mass spectrometric interrogation of thioester-bound intermediates in the initial stages of epothilone biosynthesis Journal Article

Chem Biol, 11 (3), pp. 327-35, 2004, ISSN: 1074-5521 (Print) 1074-5521 (Linking).

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Kelleher, N L

Top-down proteomics Journal Article

Anal Chem, 76 (11), pp. 197A-203A, 2004, ISSN: 0003-2700 (Print) 0003-2700 (Linking).

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LeDuc, R D; Taylor, G K; Kim, Y B; Januszyk, T E; Bynum, L H; Sola, J V; Garavelli, J S; Kelleher, N L

ProSight PTM: an integrated environment for protein identification and characterization by top-down mass spectrometry Journal Article

Nucleic Acids Res, 32 (Web Server issue), pp. W340-5, 2004, ISSN: 1362-4962 (Electronic) 0305-1048 (Linking).

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McLoughlin, S M; Kelleher, N L

Kinetic and regiospecific interrogation of covalent intermediates in the nonribosomal peptide synthesis of yersiniabactin Journal Article

J Am Chem Soc, 126 (41), pp. 13265-75, 2004, ISSN: 0002-7863 (Print) 0002-7863 (Linking).

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Meng, F; Du, Y; Miller, L M; Patrie, S M; Robinson, D E; Kelleher, N L

Molecular-level description of proteins from saccharomyces cerevisiae using quadrupole FT hybrid mass spectrometry for top down proteomics Journal Article

Anal Chem, 76 (10), pp. 2852-8, 2004, ISSN: 0003-2700 (Print) 0003-2700 (Linking).

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Patrie, S M; Charlebois, J P; Whipple, D; Kelleher, N L; Hendrickson, C L; Quinn, J P; Marshall, A G; Mukhopadhyay, B

Construction of a hybrid quadrupole/Fourier transform ion cyclotron resonance mass spectrometer for versatile MS/MS above 10 kDa Journal Article

J Am Soc Mass Spectrom, 15 (7), pp. 1099-108, 2004, ISSN: 1044-0305 (Print) 1044-0305 (Linking).

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Pesavento, J J; Kim, Y B; Taylor, G K; Kelleher, N L

Shotgun annotation of histone modifications: a new approach for streamlined characterization of proteins by top down mass spectrometry Journal Article

J Am Chem Soc, 126 (11), pp. 3386-7, 2004, ISSN: 0002-7863 (Print) 0002-7863 (Linking).

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Tseng, C C; McLoughlin, S M; Kelleher, N L; Walsh, C T

Role of the active site cysteine of DpgA, a bacterial type III polyketide synthase Journal Article

Biochemistry, 43 (4), pp. 970-80, 2004, ISSN: 0006-2960 (Print) 0006-2960 (Linking).

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Xie, L; Miller, L M; Chatterjee, C; Averin, O; Kelleher, N L; van der Donk, W A

Lacticin 481: in vitro reconstitution of lantibiotic synthetase activity Journal Article

Science, 303 (5658), pp. 679-81, 2004, ISSN: 1095-9203 (Electronic) 0036-8075 (Linking).

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Patrie, S M; Robinson, D E; Meng, F; Kelleher, N L

Strategies for automating top down proteomics with Q-FT ICR MS Journal Article

Int. J. Mass Spectrom, 234 , pp. 175-184, 2004.

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2003

He, S; Bauman, D; Davis, J S; Loyola, A; Nishioka, K; Gronlund, J L; Reinberg, D; Meng, F; Kelleher, N; McCafferty, D G

Facile synthesis of site-specifically acetylated and methylated histone proteins: reagents for evaluation of the histone code hypothesis. Journal Article

Proc Natl Acad Sci U S A, 100 (21), pp. 12033-8, 2003.

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Charlebois, J P; Patrie, S M; Kelleher, N L

Electron capture dissociation and 13C,15N depletion for deuterium localization in intact proteins after solution-phase exchange Journal Article

Anal Chem, 75 (13), pp. 3263-6, 2003, ISSN: 0003-2700 (Print) 0003-2700 (Linking).

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Mazur, M T; Walsh, C T; Kelleher, N L

Site-specific observation of acyl intermediate processing in thiotemplate biosynthesis by fourier transform mass spectrometry: the polyketide module of yersiniabactin synthetase Journal Article

Biochemistry, 42 (46), pp. 13393-400, 2003, ISSN: 0006-2960 (Print) 0006-2960 (Linking).

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Taylor, G K; Kim, Y B; Forbes, A J; Meng, F; McCarthy, R; Kelleher, N L

Web and database software for identification of intact proteins using "top down" mass spectrometry Journal Article

Anal Chem, 75 (16), pp. 4081-6, 2003, ISSN: 0003-2700 (Print) 0003-2700 (Linking).

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2002

Johnson, J R; Meng, F; Forbes, A J; Cargile, B J; Kelleher, N L

Fourier-transform mass spectrometry for automated fragmentation and identification of 5-20 kDa proteins in mixtures Journal Article

Electrophoresis, 23 (18), pp. 3217-23, 2002, ISSN: 0173-0835 (Print) 0173-0835 (Linking).

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Meng, F; Cargile, B J; Patrie, S M; Johnson, J R; McLoughlin, S M; Kelleher, N L

Processing complex mixtures of intact proteins for direct analysis by mass spectrometry Journal Article

Anal Chem, 74 (13), pp. 2923-9, 2002, ISSN: 0003-2700 (Print) 0003-2700 (Linking).

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Valaskovic, G A; Kelleher, N L

Miniaturized formats for efficient mass spectrometry-based proteomics and therapeutic development Journal Article

Curr Top Med Chem, 2 (1), pp. 1-12, 2002, ISSN: 1568-0266 (Print) 1568-0266 (Linking).

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2001

Forbes, A J; Mazur, M T; Patel, H M; Walsh, C T; Kelleher, N L

Toward efficient analysis of >70 kDa proteins with 100% sequence coverage Journal Article

Proteomics, 1 (8), pp. 927-33, 2001, ISSN: 1615-9853 (Print) 1615-9853 (Linking).

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Meng, F; Cargile, B J; Miller, L M; Forbes, A J; Johnson, J R; Kelleher, N L

Informatics and multiplexing of intact protein identification in bacteria and the archaea Journal Article

Nat Biotechnol, 19 (10), pp. 952-7, 2001, ISSN: 1087-0156 (Print) 1087-0156 (Linking).

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2000

Kelleher, N L

From primary structure to function: biological insights from large-molecule mass spectra Journal Article

Chem Biol, 7 (2), pp. R37-45, 2000, ISSN: 1074-5521 (Print) 1074-5521 (Linking).

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Zubarev, R A; Horn, D M; Fridriksson, E K; Kelleher, N L; Kruger, N A; Lewis, M A; Carpenter, B K; McLafferty, F W

Electron capture dissociation for structural characterization of multiply charged protein cations Journal Article

Anal Chem, 72 (3), pp. 563-73, 2000, ISSN: 0003-2700 (Print) 0003-2700 (Linking).

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1999

Kelleher, N L; Lin, H Y; Valaskovic, G A; Aaserud, D J; Fridriksson, E K; McLafferty, F W

Top Down versus Bottom Up Protein Characterization by Tandem High-Resolution Mass Spectrometry Journal Article

J. Am. Chem. Soc. , 121 (4), pp. 806-812, 1999.

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Kelleher, N L; Hendrickson, C L; Walsh, C T

Posttranslational heterocyclization of cysteine and serine residues in the antibiotic microcin B17: distributivity and directionality Journal Article

Biochemistry, 38 (47), pp. 15623-30, 1999, ISSN: 0006-2960 (Print) 0006-2960 (Linking).

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Kelleher, N L; Zubarev, R A; Bush, K; Furie, B; Furie, B C; McLafferty, F W; Walsh, C T

Localization of labile posttranslational modifications by electron capture dissociation: the case of gamma-carboxyglutamic acid Journal Article

Anal Chem, 71 (19), pp. 4250-3, 1999, ISSN: 0003-2700 (Print) 0003-2700 (Linking).

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Milne, J C; Roy, R S; Eliot, A C; Kelleher, N L; Wokhlu, A; Nickels, B; Walsh, C T

Cofactor requirements and reconstitution of microcin B17 synthetase: a multienzyme complex that catalyzes the formation of oxazoles and thiazoles in the antibiotic microcin B17 Journal Article

Biochemistry, 38 (15), pp. 4768-81, 1999, ISSN: 0006-2960 (Print) 0006-2960 (Linking).

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Shaw-Reid, C A; Kelleher, N L; Losey, H C; Gehring, A M; Berg, C; Walsh, C T

Assembly line enzymology by multimodular nonribosomal peptide synthetases: the thioesterase domain of E. coli EntF catalyzes both elongation and cyclolactonization Journal Article

Chem Biol, 6 (6), pp. 385-400, 1999, ISSN: 1074-5521 (Print) 1074-5521 (Linking).

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Roy, Sinha R; Kelleher, N L; Milne, J C; Walsh, C T

In vivo processing and antibiotic activity of microcin B17 analogs with varying ring content and altered bisheterocyclic sites Journal Article

Chem Biol, 6 (5), pp. 305-18, 1999, ISSN: 1074-5521 (Print) 1074-5521 (Linking).

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Zubarev, R A; Fridriksson, E K; Horn, D M; Kelleher, N L; Kruger, N A; Lewis, M A; Carpenter, B K; Mclafferty, F W

Electron Capture Dissociation Produces Many More Protein Backbone Cleavages Than Collisional and IR Excitation Journal Article

Mass Spec in Bio & Med, pp. 111-120, 1999, ISBN: 978-1-4684-9829-5.

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1998

Kelleher, N L; Belshaw, P J; Walsh, C T

Regioselectivity and chemoselectivity analysis of oxazole and thiazole ring formation by the peptide-heterocyclizing microcin B17 synthetase using high-resolution MS/MS Journal Article

J. Am. Chem. Soc., 120 (37), pp. 9716-9717, 1998, ISSN: 00027863.

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McLafferty, F W; Guan, Z; U, Haupts ; Wood, T D; Kelleher, N L

Gaseous conformational structures of cytochrome c Journal Article

J. Am. Chem. Soc., 120 (19), pp. 4732-4740, 1998, ISSN: 00027863.

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Belshaw, P J; Roy, R S; Kelleher, N L; Walsh, C T

Kinetics and regioselectivity of peptide-to-heterocycle conversions by microcin B17 synthetase Journal Article

Chem Biol, 5 (7), pp. 373-84, 1998, ISSN: 1074-5521 (Print) 1074-5521 (Linking).

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Kelleher, N L; Taylor, S V; Grannis, D; Kinsland, C; Chiu, H J; Begley, T P; McLafferty, F W

Efficient sequence analysis of the six gene products (7-74 kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry Journal Article

Protein Sci, 7 (8), pp. 1796-801, 1998, ISSN: 0961-8368 (Print) 0961-8368 (Linking).

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Kinsland, C; Taylor, S V; Kelleher, N L; McLafferty, F W; Begley, T P

Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis Journal Article

Protein Sci, 7 (8), pp. 1839-42, 1998, ISSN: 0961-8368 (Print) 0961-8368 (Linking).

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McLafferty, F W; Kelleher, N L; Begley, T P; Fridriksson, E K; Zubarev, R A; Horn, D M

Two-dimensional mass spectrometry of biomolecules at the subfemtomole level Journal Article

Curr Opin Chem Biol, 2 (5), pp. 571-8, 1998, ISSN: 1367-5931 (Print) 1367-5931 (Linking).

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Milne, J C; Eliot, A C; Kelleher, N L; Walsh, C T

ATP/GTP hydrolysis is required for oxazole and thiazole biosynthesis in the peptide antibiotic microcin B17 Journal Article

Biochemistry, 37 (38), pp. 13250-61, 1998, ISSN: 0006-2960 (Print) 0006-2960 (Linking).

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365 entries « 7 of 8 »